Phosphoester hydrolysis by cerium(IV)-thiacalix[4]arene complexes and its application to immunoassay |
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Authors: | Hiroaki Matsumiya Hiroko Nakamura Masataka Hiraide |
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Affiliation: | (1) Department of Molecular Design and Engineering, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan |
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Abstract: | Thiacalix[4]arenetetrasulfonate was treated with Ce(IV) in water at pH 9.5 to give novel phosphoester-hydrolyzing complexes. The dinuclear Ce(IV) complex promoted the hydrolysis of p-nitrophenyl phosphate with a turnover frequency of 6.8 h−1 at 50 °C, showing fourfold higher activity than the mononuclear complex. The dinuclear complex was readily immobilized onto an antibody by simply mixing them in water, hence its phosphatase-like activity was applied to the color-developing reaction in immunoassay. The model assay using an antibody labeled with the dinuclear complex allowed the detection of as little as 10 ng mL−1 of a tumor marker, Bence–Jones protein, in a 96-well microtiter plate format. Analysis of urine for Bence–Jones protein was performed by the proposed method. |
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Keywords: | Thiacalixarene Cerium Phosphoester hydrolysis Immunoassay Bence– Jones protein |
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