High-performance purification of quaternary alkaloids from Corydalis yanhusuo W. T. Wang using a new polar-copolymerized stationary phase |
| |
Authors: | Wang Chaoran Guo Zhimou Zhang Jing Zeng Jing Zhang Xiuli Liang Xinmiao |
| |
Institution: | Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P. R. China. |
| |
Abstract: | An efficient method for purification of alkaloids from Corydalis yanhusuo W. T. Wang using HPLC was developed, featuring a polar-copolymerized stationary phase named C18HCE. As ionizable solutes, the crude alkaloid sample often suffered from serious peak tailing problem on conventional RP-LC columns, and the separation would rapidly became destroyed with the increasing of load amount. However, on the new stationary phase, good peak shapes (asymmetry factor <1.5) as well as good loadability were easily obtained in a commonly used acidic mobile phase condition. The loading amount could reach 10 mg per injection on an analytical C18HCE column for laboratory-scale purification. About 6.8 mg of palmatine (HPLC purity >98%) and 44.4 mg of dehydrocorydaline (HPLC purity >98%) were rapidly derived from 200 mg of the crude alkaloid sample, and the recoveries of these two compounds were 76.5 and 81.7%, respectively. The purified alkaloids were characterized by comparing retention times with standard compounds as well as (1)H-NMR data. The new method is simple and high yielding, and it may provide a promising tool for purification of alkaloids as well as other alkaline compounds. |
| |
Keywords: | Alkaloids Corydalis yanhusuo W T Wang HPLC Polar‐copolymerized Purification |
本文献已被 PubMed 等数据库收录! |
|