Enhanced Performance of Pulsed Q Collision Induced Dissociation-Based Peptide Identification on a Dual-Pressure Linear Ion Trap |
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Authors: | Christopher Lößner Walter Blackstock Jayantha Gunaratne |
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Affiliation: | (1) Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, 61 Biopolis Drive, Singapore, Singapore, 138673; |
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Abstract: | Pulsed Q collision induced dissociation (PQD) was introduced for isobaric tag quantification on linear ion traps to circumvent the problem of the low-mass cut-off for collision induced dissociation (CID). Unfortunately, fragmentation efficiency is compromised and PQD has found limited use for identification as well as quantification. We demonstrate that PQD has a comparable peptide identification performance to CID on dual-pressure linear ion traps, opening the potential for wider use of isobaric tag quantification on this new generation of linear ion traps. |
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