Advances in amino acid analysis |
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Authors: | Hannelore Kaspar Katja Dettmer Wolfram Gronwald Peter J Oefner |
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Institution: | (1) Institute of Functional Genomics, University of Regensburg, 93053 Regensburg, Germany |
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Abstract: | Amino acids are important targets for metabolic profiling. For decades, amino acid analysis has been accomplished by either
cation-exchange or reversed-phase liquid chromatography coupled to UV absorbance or fluorescence detection of pre-column or
post-column-derivatized amino acids. Recent years have seen great progress in the development of direct-infusion or hyphenated
mass spectrometry in the analysis of free amino acids in physiological fluids, because mass spectrometry not only matches
optical detection in sensitivity, but also offers superior selectivity. The advent of cryo-probes has also brought NMR spectroscopy
within the detection limits required for the analysis of free amino acids. But there is still room for further improvement,
including expansion of the analyte spectrum, reduction of sample preparation and analysis time, automation, and synthesis
of affordable isotope standards.
Figure Fully automated gas chromatography-mass spectrometry analysis of amino acids. |
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Keywords: | Amino acids Quantitative analysis Chromatography Mass spectrometry NMR spectroscopy Derivatization |
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