Biophysical Properties of Phenyl Succinic Acid Derivatised Hyaluronic Acid |
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Authors: | Maria?Teresa?Neves-Petersen S?ren?Klitgaard Esben?Skovsen Steffen?B?Petersen Kristoffer?T?mmeraas Khadija?Schwach-Abdellaoui |
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Institution: | (1) NanoBiotechnology Group, Department of Physics and Nanotechnology, Aalborg University, Skjernvej 4A, Aalborg, Denmark;(2) The Institute for Lasers, Photonics and Biophotonics, University at Buffalo, The State University of New York Buffalo, New York, NY 14260-3000, USA;(3) Novozymes, Krogshoejvej 36, Bagsvaerd, 2880, Denmark |
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Abstract: | Modification of hyaluronic acid (HA) with aryl succinic anhydrides results in new biomedical properties of HA as compared
to non-modified HA, such as more efficient skin penetration, stronger binding to the skin, and the ability to blend with hydrophobic
materials. In the present study, hyaluronic acid has been derivatised with the anhydride form of phenyl succinic acid (PheSA).
The fluorescence of PheSA was efficiently quenched by the HA matrix. HA also acted as a singlet oxygen scavenger. Fluorescence
lifetime(s) of PheSA in solution and when attached to the HA matrix has been monitored with ps resolved streak camera technology.
Structural and fluorescence properties changes induced on HA-PheSA due to the presence of singlet oxygen were monitored using
static light scattering (SLS), steady state fluorescence and ps time resolved fluorescence studies. SLS studies provided insight
into the depolymerisation kinetics of PheSA derivatised HA matrix in the presence of singlet oxygen. Time resolved fluorescence
studies grave insight into the dynamics of the reaction mechanisms induced on HA-PheSA by singlet oxygen. These studies provided
insight into the medical relevance of PheSA derivatised HA: its capacity of scavenging singlet oxygen and of quenching PheSA
fluorescence. These studies revealed that HA-PheSA is a strong quencher of electronic excited state PheSA and acts as a scavenger
of singlet oxygen, thus medical applications of this derivatised form of HA may protect tissues and organs, such as skin,
against reactive oxygen species damage. |
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