流动注射化学发光法及光度法用于巴戟天提取液抗氧化活性的研究

将中药巴戟天提取液加入鲁米诺-H₂O₂-CuSO₄化学发光体系，测量其发光强度, 根据系统化学发光被抑制的程度可以评价巴戟天的抗氧化活性，并将巴戟天与抗坏血酸的抗自由基活性进行了对比实验，实验证明巴戟天提取液具有明显的抗自由基活性;采用分光光度法测定体系吸光度的变化来研究巴戟天对OH·及O₂-·自由基的清除作用，以抗坏血酸作阳性对照，结果发现：巴戟天对OH·及O₂-·自由基均有良好的清除作用，结果较为满意。提示中药巴戟天可作为潜在的具有抗氧化活性的天然药物。

Determination of Antioxidation of the Extract from Chinese Medicine Morinda Officinalis How by Flow Injection Chemiluminescence and Spectroscopy

Flow injection chemiluminescence analysis was used to determine the antioxidation activity of extract from Morinda officinalis How. The determination was based on the inhibition effect of Morinda officinalis How extraction in the chemiluminescence reaction of luminol - H_2O_2-CuSO_4 system, and vitamin C was used as positive control. It was showed that Morinda officinalis How original liquid has obvious anti-radical activity. Spectroscopy was applied to determine the inhibition ratio of Morinda officinalis How extraction for the superoxide anion and hydroxy radicals in the special system. It was shown that the extraction from Morinda officinalis How could obviously scavenge superoxide anion and hydroxy radicals. It is suggested that the Chinese Medicine Morinda Officinalis How is a potential antioxidation activity medicine.