| 花生再生和转化体系的初步研究 |
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| 引用本文： | 庄振宏,朱锦懋,陈由强,叶冰莹,林荣华,代容春.花生再生和转化体系的初步研究[J].福建师范大学学报(自然科学版),2001,17(4):88-92. |
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| 作者姓名： | 庄振宏 朱锦懋 陈由强 叶冰莹 林荣华 代容春 |
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| 作者单位： | 福建师范大学生物工程学院, |
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| 基金项目： | 福建省科技厅国际合作基金资助项目 ( 2 0 0 I0 0 7)，福建省科委资助项目 ( K980 16 ) |
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| 摘 要： | 以4d龄的泉花10号花生小叶伤为外植体，从25种培养基中筛选出MS＋AgNO3(1.0) 6-BA5.0 NAA2.5培养基为最佳长芽点培养基，MS＋AgNO3(1.0) 6-BA5.0 NAA1.0培养基为最佳长愈伤组织培养基；以4d龄小叶，子叶，胚轴，及子叶与胚轴相连处为外植体在MS＋AgNO3(1.0) 6-BA5.0 NAA2.5培养基上培养，结果小叶是诱导长芽的最佳外植体；研究还发现儿茶酚对农杆菌侵染力有较大的影响。
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| 关 键 词： | 花生 小叶 遗传转化 转基因再生植株 外植体 组织培养 油料作物 |
| 文章编号： | 1000-5277（2001）04-0088-05 |
| 修稿时间： | 2001年3月1日 |
The Study on the Regeneration System and Transformation System for Arachis hyqogaea L. |
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| ZHUANG Zhen-hong,ZHU Jin-mao,CHEN You-qiang,YE Bing-ying,LIN Rong-hua,DAI Rong-cun.The Study on the Regeneration System and Transformation System for Arachis hyqogaea L.[J].Journal of Fujian Teachers University(Natural Science),2001,17(4):88-92. |
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| Authors: | ZHUANG Zhen-hong ZHU Jin-mao CHEN You-qiang YE Bing-ying LIN Rong-hua DAI Rong-cun |
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| Abstract: | The medium of MS+AgNO 3(1.0)+6-BA 5.0+NAA 2.5 which was singled out from 25 different media was the best medium for leaflet which explanted from 4-day-old seedlings of peanut cultivar Quanhua No.10 to produce bud primordial, and AgNO 3(1.0)+6-BA 5.0+NAA 1.0+MS was the best medium for producing callos. Comparing to cotyledon,plumular axis and the joint of cotyledon and plumular axis, which were all cultivated on the medium of MS+AgNO 3(1.0)+6-BA 5.0+NAA 2.5, leaflet of this kind of peanut was the best explanting organ for organagenesis. This paper also primarily studied on the effect of catechol to the capacity of agrobacterium transformation for peanut. |
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| Keywords: | Arachis hyqogaea L leaflet regeneration transformation |
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