| 镧系络合物的双向螯合剂--BCPDA的性质研究 |
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| 引用本文： | 潘利华,曾春慧,关铭,赵超,林敏,谢文兵. 镧系络合物的双向螯合剂--BCPDA的性质研究[J]. 光谱学与光谱分析, 2003, 23(5): 903-906 |
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| 作者姓名： | 潘利华 曾春慧 关铭 赵超 林敏 谢文兵 |
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| 作者单位： | 中国科学院长春应用化学研究所,国家电化学光谱分析研究中心,稀土化学和物理开放实验室,吉林,长春,130022;吉林大学药学院,吉林,长春,130021 |
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| 基金项目： | 国家自然科学基金(No.30070714)资助项目 |
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| 摘 要： | 对镧系络合物的双向螯合剂——4,7-二氯磺酰基苯基-1,10-菲咯啉-2,9-二羧酸(BCPDA)合成、表征和性质研究表明:1.通过IR光谱、1H NMR、熔点、元素分析证明其结构正确。2.BCPDA可溶于几种不同溶剂。3.几种体系的BCPDA溶液所得到的吸收光谱基本相同,且BCPDA的浓度与吸光度在1.00-1.20×10~2μmol·L-1范围内呈正比。4.BCPDA标记蛋白质和铕离子与BCPDA螯合连接实验证实了BCPDA的双向功能。
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| 关 键 词： | 时间分辨荧光免疫分析 螯合剂 BCPDA |
| 文章编号： | 1000-0593(2003)05-0903-04 |
| 修稿时间： | 2001-11-05 |
Study on the Properties of BCPDA |
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| PAN Li-hua,ZENG Chun-hui,GUAN Ming,ZHAO Chao,LIN Min,XIE Wen-bing.Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,National Analytical Research Center of Electrochemistry and Spectroscopy,Laboratory of Rare Earth Chemistry and Physics,Changchun ,China.Pharmacology College of Jilin University,Changchun ,China. Study on the Properties of BCPDA[J]. Spectroscopy and Spectral Analysis, 2003, 23(5): 903-906 |
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| Authors: | PAN Li-hua ZENG Chun-hui GUAN Ming ZHAO Chao LIN Min XIE Wen-bing.Changchun Institute of Applied Chemistry Chinese Academy of Sciences National Analytical Research Center of Electrochemistry Spectroscopy Laboratory of Rare Earth Chemistry Physics Changchun China.Pharmacology College of Jilin University Changchun China |
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| Affiliation: | PAN Li-hua,ZENG Chun-hui,GUAN Ming,ZHAO Chao,LIN Min,XIE Wen-bing1.Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,National Analytical Research Center of Electrochemistry and Spectroscopy,Laboratory of Rare Earth Chemistry and Physics,Changchun 130022,China2.Pharmacology College of Jilin University,Changchun 130021,China |
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| Abstract: | A new chelate-4,7-bis (chlorosulfophenyl)-1,10-phenanthroline-2,9-dicaboxylic acid (BCPDA) was synthesized for time-resolved fluorescence immunoassay (TRFIA). Its structure and purity were confirmed by means of IR, 1H NMR, melt point and element analysis. Also, some of the BCPDA's properties, such as absorption spectra, emission spectra and fluorescence lifetime were discussed. A BCPDA-protein was formed when BCPDA reacted with protein under relative mild condition. A BCPDA-Eu3 chelate was formed when BCPDA reacted with EuCl2 under certain condition. The BCPDA-Eu3 can emit relatively strong fluorescence. The fluorescence lifetime of Eu3 -BCPDA-protein was measured as well. |
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| Keywords: | Time-resolved fluorescence immunoassay BCPDA property Chelate |
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