Synthesis and characterization of a new fluorescent probe for reactive oxygen species |
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| Authors: | Heyne Belinda Beddie Chad Scaiano J C |
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| Affiliation: | University of Ottawa, Department of Chemistry, 10 Marie Curie, Ottawa, ON K1N 6N5, Canada. |
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| Abstract: | We report the development of a new fluorescence sensor for reactive oxygen species (ROS) based on a benzofurazan structure. The sensor, NBFhd, is initially non-fluorescent and reacts with peroxyl radicals by hydrogen transfer in an aqueous medium under neutral conditions to release the fluorescent N-methyl-4-amino-7-nitrobenzofurazan (NBF) and 1,4-benzoquinone. NBFhd shows excellent contrast and no interference in the region of cell autofluorescence and is a new tool to detect ROS in homogeneous and biological systems. |
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