| 用紫外分光光度法和电位滴定法测定几种植物激素的电离常数 |
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| 引用本文： | 朱金花,徐辉,东莹莹,李德亮,刘绣华.用紫外分光光度法和电位滴定法测定几种植物激素的电离常数[J].化学研究,2013(3):293-298. |
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| 作者姓名： | 朱金花 徐辉 东莹莹 李德亮 刘绣华 |
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| 作者单位： | 河南大学化学化工学院;河南大学天然药物与免疫工程重点实验室 |
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| 基金项目： | 河南大学校内科研基金重点项目(2010ZRZD04) |
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| 摘 要： | 分别采用紫外分光光度法和电位滴定法测定了脱落酸、3-吲哚乙酸、赤霉素、水杨酸等几种植物激素的电离常数,对比分析了两种测试方法的原理、测试步骤和数据处理方法.结果表明,采用两种方法测定的四种样品的电离常数与文献报道值一致,且测量结果的重现性较好;这表明紫外分光光度法和电位滴定法可方便地用于测定有机化合物的电离常数.
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| 关 键 词： | 植物激素 电离常数 紫外分光光度法 电位滴定 测定 |
Determination of ionization constant of several plant hormones by ultraviolet spectrophotometry and potentiometric titration |
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| ZHU Jin-hua,XU Hui,DONG Ying-ying,LI De-liang,LIU Xiu-hua.Determination of ionization constant of several plant hormones by ultraviolet spectrophotometry and potentiometric titration[J].Chemical Research,2013(3):293-298. |
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| Authors: | ZHU Jin-hua XU Hui DONG Ying-ying LI De-liang LIU Xiu-hua |
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| Institution: | 1,2*(1.College of Chemistry and Chemical Engineering,Henan University,Kaifeng 475004,Henan,China; 2.Key Laboratory of Natural Drug and Immune Engineering of Henan Province,Henan University, Kaifeng 475004,Henan,China) |
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| Abstract: | Ultraviolet spectrophotometric method and potentiometric titration method were sep- arately adopted to determine the ionization constant of several plant hormones including abscisic acid, 3dndole acetic acid, gibberellic acid and salicylic acid. The principle, procedure, and da- ta-process of the two methods were discussed and compared. Results indicate that the ioniza- tion constants of the ~our samples determined by the two methods are in agreement with that reported in literatures, and they have good reproducibilities. This means that both ultraviolet spectrophotometric method and potentiometric titration method can be well adopted to deter- mine the ionization constants of organic compounds. |
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| Keywords: | plant hormone ionization constant ultraviolet spectrophotometry potentiometric titration determination |
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