Electrospun poly(ethylene oxide)/chitosan nanofibers with cellulose nanocrystals as support for cell culture of 3T3 fibroblasts |
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| Authors: | Daniela M Ridolfi Ana Paula Lemes Sheila de Oliveira Giselle Z Justo Marcelly V Palladino Nelson Durán |
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| Institution: | 1.Institute of Chemistry,Universidade Estadual de Campinas,Campinas,Brazil;2.Institute of Science and Technology,Universidade Federal de S?o Paulo,S?o José dos Campos,Brazil;3.Department of Biochemistry (Campus S?o Paulo),Universidade Federal de S?o Paulo,S?o Paulo,Brazil;4.Department of Biological Sciences (Campus Diadema),Universidade Federal de S?o Paulo,S?o Paulo,Brazil;5.NANOBIOSS (MCTI), Institute of Chemistry,Universidade Estadual de Campinas,Campinas,Brazil;6.Brazilian National Nanotechnology Laboratory (LNNano-CNPEM),Campinas,Brazil |
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| Abstract: | Chitosan/poly(ethylene oxide) (PEO) (5:1) nanofibers with cellulose nanocrystals (CNCs) were produced using an electrospinning technique. The addition of CNCs to the chitosan/PEO solutions allowed the production of uniform fibers (without beads) with a high proportion of chitosan. The fiber diameters were influenced by the concentration of CNCs in the chitosan/PEO solutions. The solutions containing 10% (w/w) of CNCs produced thinner fibers compared to solutions containing 5% (w/w) of CNCs. Thermogravimetric analysis indicated that the nanofibers were thermally stable, despite the CNCs having an effect on the PEO decomposition. Results from the cell assay in cultures of 3T3 fibroblasts indicated that the chitosan/PEO nanofibers (with 10% CNCs) promoted cell attachment with changes in the cytoskeletal organization. The results obtained in this work highlight the favorable effect of CNCs in electrospinning of chitosan/PEO. As expected, the influence of nanofibers on 3T3 fibroblasts F-actin and β-tubulin network revealed alterations in cytoskeleton, leading to changes in cell morphology and spreading. |
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