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Characterization of spherical particles using high-order neural networks and scanning flow cytometry |
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| Authors: | Vladimir V. Berdnik Alexander Shvalov Valeri Maltsev Valery A. Loiko |
| Affiliation: | a Institute for Aerospace technology, Lipatov str., 2, 420075 Kazan, Russia b Sobolev Institute of Mathematics, Siberian Branch of the Russian Academy of Sciences, 4 Acad. Koptyug avenue, 630090 Novosibirsk, Russia c Novosibirsk State University, Pirogova Str. 2, 630090 Novosibirsk, Russia d Institute of Chemical Kinetics and Combustion, Siberian Branch of the Russian Academy of Sciences, Institutskaya Str. 3, 630090 Novosibirsk, Russia e B.I. Stepanov Institute of Physics of the National Academy of Sciences of Belarus, F. Scaryna avenue, 68, 220072 Minsk, Belarus |
| Abstract: | We retrieve the radius R, real n and imaginary k parts of the refractive index of homogeneous spherical particles using angular distribution of the light-scattering intensity. To solve the inverse light-scattering problem we use a high-order neural-network technique. The effect of network parameters on optimization is examined. The technique is evaluated for noise-corrupted input data at 0.6 μm<R<10.6 μm, 1.02<n<1.38, and 0<k<0.03. The errors of retrieval for nonabsorbing particles do not exceed 0.05 μm for radius and 0.015 for refractive index. The experimental verification is fulfilled by experimental data retrieved by means of a scanning flow cytometer. The light-scattering profiles of polystyrene beads and spherized red blood cells are processed with the high-order neural networks and a non-linear regression at Mie theory. The parameters retrieved by the high-order neural networks correlate well with the parameters retrieved by the least-square method. |
| Keywords: | Neural networks Particle sizing Inverse light scattering problem |
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