A selective turn-on fluorescent sensor for imaging copper in living cells |
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Authors: | Zeng Li Miller Evan W Pralle Arnd Isacoff Ehud Y Chang Christopher J |
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Institution: | Department of Chemistry, University of California, Berkeley, CA 94720, USA. |
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Abstract: | We present the synthesis, properties, and biological applications of Coppersensor-1 (CS1), a new water-soluble, turn-on fluorescent sensor for intracellular imaging of copper in living biological samples. CS1 utilizes a BODIPY reporter and thioether-rich receptor to provide high selectivity and sensitivity for Cu+ over other biologically relevant metal ions, including Cu2+, in aqueous solution. This BODIPY-based probe is the first Cu+-responsive sensor with visible excitation and emission profiles and gives a 10-fold turn-on response for detecting this ion. Confocal microscopy experiments further establish that CS1 is membrane-permeable and can successfully monitor intracellular Cu+ levels within living cells. |
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