代谢组学方法分析鸡胚胎发育过程中脑代谢物

本文应用高分辨魔角旋转核磁共振（HR-MAS NMR）方法对发育过程中（孵化12天至出壳后1天）鸡胚胎大脑、小脑和视叶3种脑组织中的代谢物含量进行了测定，并利用模式识别方法对整体代谢特征进行了归类分析. 结果表明：胚胎发育过程中不同脑区的代谢物组成存在差异，可能与这些脑区的功能及发育特征等的不同有关. 在NMR方法检测到的多种小分子代谢物中，γ-氨基丁酸、N-乙酰天冬氨酸、牛磺酸、肌醇以及胆碱等在鸡胚胎脑组织中的分布有区域性差异，这些差异也是区分大脑、小脑和视叶组织的特征性代谢物.

Dynamic Changes of Regional Metabolic Profiles in Chick Embryo Brain during Development Studied by An NMR-based Metabonomic Approach

An NMR based metabonomic approach was applied to characterize the dynamic changes of regional metabolic profiles in the brain of chick embryo during development from incubation day 12 to postnatal day 1. The NMR spectra of intact brain tissues from the cerebrum, cerebellum and optic lobes of chick embryo were acquired using high-resolution magic-angle-spinning (HR-MAS) ¹H NMR spectroscopy, and statistically classified using principle component analysis (PCA). The levels of 25 metabolites were measured quantitatively. The results indicated that the metabolic profiles were different among each other in three brain regions during development. The main contributors to the classification were the concentrations of GABA, NAA, taurine and myo-inositol.