Genotyping using single nucleotide polymorphism, fluorescence spectroscopy and pattern recognition |
| |
Authors: | Brereton Richard G Devonshire Martin |
| |
Affiliation: | School of Chemistry, University of Bristol, Cantock's Close, Bristol BS8 1TS, UK. |
| |
Abstract: | This paper describes a method for genetic screening using single nucleotide polymorphism. Fluorescence spectra with an excitation frequency of 488 nm are recorded over a range of 550 to 660 nm of fragments of human DNA together with two fluorescent probe dyes attached to specific primers, one for each type of allele and a background dye, prepared using the Taqman reaction. The fluorescence spectra are monitored and principal components analysis used to separate spectra into three groups, which are visually identified as allele 1 (wild type), allele 2 (mutant) and mixed allele by comparison to reference samples. Malahanobis distance using 4 principal components are used to correctly classify samples into groups. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|