| 胰岛素类似物氨基酸残基质子自旋体系的二维谱模拟 |
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| 引用本文： | 华庆新. 胰岛素类似物氨基酸残基质子自旋体系的二维谱模拟[J]. 波谱学杂志, 1990, 7(1): 1-9 |
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| 作者姓名： | 华庆新 |
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| 作者单位： | 中国科学院生物物理所 |
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| 基金项目： | 国家自然科学基金委员会(生-85-114)，美国国立健康研究院(NIH DK 34035)基金 |
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| 摘 要： | 以MARS程序对胰岛素类似物的残基质子自旋体系作计算机模拟,由模拟谱与B链C端去五肽胰岛素、S-磺酸型胰岛素A、B链的二维相关谱的比较,表明模拟谱与实测谱交叉峰图形及精细结构十分相似,验证了实测谱所作残基质子自旋体系的鉴别,同时也表明此程序可广泛应用于蛋白质等生物分子的二维谱模拟。
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| 关 键 词： | 二维核磁共振 二维谱模拟 胰岛素 |
| 收稿时间： | 1988-08-16 |
SPIN SYSTEM SIMULATION OF 2D SPECTRA OF AMINO ACID RESIDUES IN INSULIN ANALOGS |
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| Hua Qingxin. SPIN SYSTEM SIMULATION OF 2D SPECTRA OF AMINO ACID RESIDUES IN INSULIN ANALOGS[J]. Chinese Journal of Magnetic Resonance, 1990, 7(1): 1-9 |
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| Authors: | Hua Qingxin |
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| Affiliation: | Institute of Biophysics, Academia Sinica |
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| Abstract: | 1H spin system simulations of 2D-COSY spectrum of amino acid residues in insulin analogs were reported by using MARS program. From the comparison of the simulation spectra with the observed 2D-COSY spectra of Dcs-( B36-B23) -insulin and S-sulfonated insulin A and B chains, the cross peak patterns and fine structrues of simulated and obverved spectra were quite similar, indicating that the1H spin system identifications of residues from the observed 2D spectra arc correct, and on the other hand, the program MARS can be extensively used for the simulations of 2D-spectra of proteins. |
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| Keywords: | 2D-NMR 2D simulation spectra Insulin. |
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