反相高效液相色谱法分离测定墨旱莲中的鳢肠醛

对墨旱莲中的有效成分鳢肠醛进行了分离和结构鉴定,并对其含量进行了分析。色谱条件:Kromasil C18柱(200 mm×4.6 mm,5 μm),流动相为乙腈-0.1%磷酸水溶液(体积比为65∶35),流速为1.0 mL/min,柱温为30 ℃,检测波长为365 nm,进样量为10 μL。结果表明,鳢肠醛的峰面积与其质量浓度有良好的线性关系(r=0.9993)。方法的加样回收率为96.7%～100.0%。该方法简便、快速、准确,可作为墨旱莲质量控制的一个有效方法。

Isolation and Determination of Ecliptal in Herba Ecliptae by Reversed-Phase High Performance Liquid Chromatography

The ecliptal was isolated from Herba Ecliptae by high performance liquid chromatography and identified by mass spectrometry and nuclear magnetic resonance (1H NMR). A method for determination of ecliptal in Herba Ecliptae was developed by using a Kromasil C18 column (200 mm x 4.6 mm, 5 microm) with a mixture of acetonitrile and 0.1% phosphoric acid solution (65:35, v/v) as mobile phase at a flow rate of 1.0 mL/min. The detection wavelength was set at 365 nm and temperature was set at 30 degrees C. The volume of injection was 10 microL. There was good linear relationship (r = 0. 9993) between the mass concentration and the peak area of ecliptal in the range of 3.0 - 60.0 microg/mL. The recovery was found to be in the range of 96.7% - 100.0%. The results of the experiments have demonstrated that the established method is rapid and simple with good accuracy and reproducibility. The method is suitable for the quality control of Herba Ecliptae from different sources.