| Abstract: | The carbon nanotubes decorated nanoplatinum (CNT‐Pt) were prepared using a chemical reduction method and a novel base electrode was constructed by intercalating CNT‐Pt on the surface of a waxed graphite electrode. The results showed that the nano‐particles of platinum at a waxed graphite electrode exhibits high catalytic activity for the reduction of hydrogen peroxide. The cholesterol oxidase (ChOx), chosen as a model enzyme, was immobilized with sol‐gel on the CNT‐Pt base electrode to construct a biosensor. The current response of the biosensor for cholesterol was very rapid (<20 s). The linear range for cholesterol measurement was 4.0×10?6 mol/L ?1.0×10?4 mol/L with a detection limit of 1.4×10?6 mol/L. The experiments also showed that the ChOx/sol‐gel/CNT‐Pt biosensor was sensitive and stable in detecting cholesterol in serum samples. |
