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Novel biomaterials derived from deoxyribozyme and NAPzyme |
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| Authors: | Yasuhide Okumoto Hiroyoshi Fujiki Junji Kawakami Shoji Nakashima Shu-ichi Nakano Tatsuo Ohmichi Daisuke Miyoshi Naoki Sugimoto |
| Institution: | 1. Department of Chemistry, Faculty of Science and Engineering and
Nippon Laser & Electronics Lab., 20-9, Sanbonmatsu-cho, Atsuta-ku, Nagoya 456-0032, Japan;2. Department of Chemistry, Faculty of Science and Engineering and;3. Department of Chemistry, Faculty of Science and Engineering and High Technology Research Center, Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501, Japan;4. High Technology Research Center, Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501, Japan |
| Abstract: | We report the potential of a small Ca2+-dependent deoxyribozyme as a novel biomaterial to distinguish RNA foldings. It is found that an immobilized deoxyribozyme using avidin-biotin interaction cleaves the target site within only single-stranded RNAs. The RNA cleavage reaction is also detected using the deoxyribozyme SPR sensor chip. Furthermore, we develop a novel NAPzyme (nucleic acid peptide deoxyribozyme) with its RNA cleavage function in the absence of divalent metal ions. |
| Keywords: | biomaterials deoxyribozyme enzymes metal ion NAPzyme (nucleic acid peptide deoxyribozyme) |