Cell sheet engineering: intelligent polymer patterned surfaces for tissue engineered liver |
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| Authors: | Masami Harimoto Masayuki Yamato Akihiko Kikuchi Teruo Okano |
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| Institution: | Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Shinjuku-ku, Tokyo 162-8666, Japan, Fax: (+81) 3-3359-6046 |
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| Abstract: | We describe a new culture system utilizing the temperature-responsive polymer grafted surface for designing of cell position and layered tissue reconstruction. Organizing of the hepatic tissue structure by controlling the culture system, that is patterned co-culture and layered cell sheet co-culture achieved by moving the cultured cells from the culture surface, resulted in regulation of the hepatocyte function. The technique for cell sheet manipulation would promote the liver tissue engineering in quality. |
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| Keywords: | cell sheet co-culture hepatocyte poly (N-isopropylacrylamide) temperature-responsive culture surface |
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