肿节风的表面增强拉曼光谱检测

提出一种基于表面增强拉曼光谱的中药材肿节风饮片的检测方法。采用柠檬酸三钠还原硝酸银制备银溶胶,以银胶纳米粒子为增强基底测得肿节风茎切片的表面增强拉曼光谱(SERS)。发现银胶直接作用于药材表面的SERS信号明显增强,肿节风茎切片SERS光谱中在637,1 176,1 309,1 476,1 612 cm^-1处都可观察到明显的拉曼特征峰。通过一阶导数拉曼光谱分析技术和对照品异嗪皮啶谱峰指认,可将获得的SERS峰位分别归属于吡喃酮环、甲氧基和酚羟基分子结构。研究结果表明,SERS技术可为肿节风和其他中草药的生产和质量监控提供一种快速、方便和直接的检测方法。

Measurement of Sarcandra Glabra Based on Surface Enhanced Raman Spectroscopy

Sarcandra glabra is one of the traditional Chinese medicine. A method was demonstrated to measure it by surface enhanced Raman spectrum (SERS) technology. Silver colloid was prepared by using aqueous reduction of silver nitrate with trisodium citrate. SERS in the samples of Sarcandra glabra was detected by taking silver nanoparticles as enhancing substrates. After disposed by the silver colloid, the intensity of the SERS signal of Sarcandra glabra stem section increased obviously. In SERS of Sarcandra glabra samples, the characteristic peaks were observed at 637, 1 176, 1 309, 1 476, 1 612 cm^-1. The SERS signal of Sarcandra glabra was assigned tentatively to some biochemical substances and molecular structure including benzopyrone ring, methoxy and phenolic hydroxyl group through a derivative spectrum of Sarcandra glabra and Isofraxiden. The results show that the SERS might provide a new, precise and convenient method for the research of tradition Chinese medicinal materials.