| 以聚二烯丙基二甲基氯化铵为探针测定人血清中总蛋白的含量 |
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| 引用本文： | 郭晔,陈艳华,田媛,高德江,邓新煜,张寒琦,于爱民,盛韧.以聚二烯丙基二甲基氯化铵为探针测定人血清中总蛋白的含量[J].吉林大学学报(理学版),2007,45(4):673-675. |
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| 作者姓名： | 郭晔 陈艳华 田媛 高德江 邓新煜 张寒琦 于爱民 盛韧 |
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| 作者单位： | 吉林大学 化学学院, 长春 130012 |
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| 摘 要： | 通过共振光散射(RLS)技术, 将聚二烯丙基二甲基氯化铵(PDDA)作为探针应用于人血清中总蛋白含量的测定. 在一定的人血清白蛋白(HSA)浓度范围(2.35×10-8-~1.17×10-6mol/L)内, HSA PDDA体系的RLS强度随HSA浓度的增加而增加, 并具有一定的线性关系. 将该方法应用于实际人血清中总蛋白含量的测定, 其结果与考马士亮蓝法得到的结果进行对比, 较为满意.
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| 关 键 词： | 共振光散射 聚二烯丙基二甲基氯化铵 人血清白蛋白 人血清 总蛋白 |
| 文章编号： | 1671-5489（2007）04-0673-03 |
| 收稿时间： | 2007-01-31 |
| 修稿时间： | 2007-01-31 |
Determination of Protein in Human Serum Albumin with Poly(diallyldimethylammonium chloride) as Probe |
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| GUO Ye,CHEN Yan-hua,TIAN Yuan,GAO De-jiang,DENG Xin-yu,ZHANG Han-qi,YU Ai-min,SHENG Ren.Determination of Protein in Human Serum Albumin with Poly(diallyldimethylammonium chloride) as Probe[J].Journal of Jilin University: Sci Ed,2007,45(4):673-675. |
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| Authors: | GUO Ye CHEN Yan-hua TIAN Yuan GAO De-jiang DENG Xin-yu ZHANG Han-qi YU Ai-min SHENG Ren |
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| Institution: | College of Chemistry, Jilin University, Changchun 130012, China |
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| Abstract: | Based on the interaction of total protein with poly(diallyldimethylammonium chloride) (PDDA), a new method to determine the total protein in human serum was developed with the resonance light scattering (RLS) technique. Under appropriate conditions, the RLS intensity at the wavelength of 320 nm is directly proportional to the protein concentration in a range of 2.35×1
0-8~1.17×10-6 mol/L. The total protein in human serum was determined with the method mentioned above. The method was also compared with Bradford
method and the results obtained were satisfactory. |
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| Keywords: | resonance light scattering poly(diallyldimethylam monium chloride) human serum albumin human serum total protein |
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