AFLP分子标记在鹀属遗传多样性分析中的初探

利用扩增片断长度多态性（AFLP）技术分析了鹀属(Emberiza)4种鹀(三道眉草鹀Emberi za cioides, 白头鹀Emberiza leucocephala, 白眉鹀Emberiza tristrami, 灰头鹀Emberiza spodocephala)基因组DNA的多态性. 在选取的39对引物中, 有11对能得到重复性好的多态性扩增条带， 每对引物扩增带数为27~76. 共得到535个标记位点, 其中429个为多态位点, 多态位点 比率达801%; 在UPGMA聚类关系图中， 4种鹀聚成一大类， 三道眉草鹀与白头鹀、 白眉鹀与灰头鹀分别两两相聚. 结果表明， AFLP可用于分析鹀属遗传多样性.

AFLP Markers Analysis of Genetic Diversity in Emberiza

The amplified fragment length polymorphism (AFLP) technique was used to assess the genomic DNA polymorphism of four species Emberiza (cioides, leucocephala, tristrami and spodocephala)Eleven pairs out of thirty nine AFLP primer pairs produced reproducible polymorphic DNA amplification patterns.The amplified bands ranged from 27~76 per primer pair. Among the 535 AFLP markers scored, 429 ones werepolymorphic. The proportion of polymorphic loci was 801%. In UPGMA cluster analysis, four buntings were grouped into one branch, in which Emberiza cioidesand Emberiza leucocephala clustered together, and Emberiza tristramis and Emberiza spodocephala were grouped closely The result indicates that it is practical to reveal genetic diversity of Emberiza with AFLP markers.