| Abstract: | Reversed‐phase liquid chromatography (RP‐HPLC) has become a powerful and widely employed technique for the analysis of a great variety of substances and, in particular, of basic compounds. These compounds are present in various areas. In pharmacy, 80% of drugs are estimated to possess a basic function. Basic compounds can strongly interact with free silanol groups on the surface of the silica particles. These ion exchange interactions produce peak tailing which affects resolution, sensitivity, and reproducibility. For these reasons, many new stationary phases have been designed to reduce access to silanol groups. The main problem facing the analyst is to effectively select the best column for a particular type of separation. In order to characterize and evaluate the properties of these packings, several tests are proposed in the literature, which can be divided into two main categories: general tests and particular tests. In this work, a particular test was developed for the characterization of base deactivated RP‐HPLC stationary phases. For this purpose, a set of 14 basic test substances was selected and five different chromatographic supports were tested with three isocratic mobile phases. Furthermore, in order to undertake a complete characterization of these supports, batch and column reproducibility were also studied. Principal Component Analysis was applied to evaluate both the performance of the test compounds and of the stationary phases. |
