| Abstract: | To extend the applicability of electrokinetic chromatography (EKC), two new types of pseudostationary phases have been introduced. A high-molecular surfactant, butyl acrylate/butyl methacrylate/methacrylic acid copolymer (BBMA) is employed as a micellar forming surfactant for miccllar electrokinetic chromatography (MEKC). The critical micelle concentration of BBMA is essentially zero, which means the micellar concentration is constant irrespective of temperature and buffer. Some characteristic features of BBMA as the pseudostationary phase for MEKC is investigated in comparison with conventional ionic surfactants. Ovomucoid and avidin, which are proteins isolated from egg white, have been found to be useful chiral selectors in affinity EKC. A few examples of the separation of enantiomers with these proteins are shown. |
