Dialysis-Chelex method for determination of exchangeable copper in human plasma |
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| Authors: | Tony I Venelinov Ian M Davies John H Beattie |
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| Institution: | (1) Department of Analytical Chemistry, Faculty of Chemistry, Sofia University, 1 J. Bourchier Blvd, 1164 Sofia, Bulgaria;(2) FRS Marine Laboratory, 375 Victoria Road, PO Box 101, Aberdeen, AB11 9DB, UK;(3) Rowett Research Institute, Greenburn Road, Buckburn, Aberdeen, AB21 9SB, UK |
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| Abstract: | A method based on dialysis in the presence of histidine, and subsequent copper adsorption and preconcentration using Chelex-100 resin, has been developed for selective extraction of exchangeable copper from blood plasma. The method was verified by comparative analysis using two different instrumental techniques, ICP–MS and AAS. The results obtained were in excellent agreement. Accurate quantification of the exchangeable copper and the firmly bound copper was achieved. The method was further validated by analysis of a lyophilised human serum certified reference material. |
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| Keywords: | Exchangeable copper Dialysis Chelex-100 ICP– MS |
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