A Simple Design to Realize Micro-column Separation by Conventional Analytical HPLC |
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Authors: | GONG Wenjun ZHANG Junxia ZHANG Yuping ZHANG Yijun TIAN Mengkui WU Dafu |
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Affiliation: | 1. Henan Institute of Science and Technology, Xinxiang, Henan 453003, China;2. Henan Provincial Institute of Food and Drug Control, Zhengzhou, Henan 450003, China |
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Abstract: | The conventional analytical HPLC was successfully developed for micro‐column separation by using a simple eluate splitting system, self‐preparation of packing column and on‐capillary column detector in our laboratory. Porous inlet frit in fused silica capillary was rapidly prepared by sintering stainless steel powders under 500 meshes for about 20 s. The use of such frits or metal meshes in capillary to retain C18 particles of chromatographic packing was demonstrated to be stable and specially robust with continuous packing and long chromatographic runs. Furthermore, the chromatographic behavior was detailedly evaluated by changing the flow rate and the percentage of mobile phase using the prepared capillary column. Under the optimal experimental conditions, baseline separation of the model analytes including thiourea, benzene, toluene, ethylbenzene was obtained with a high column efficiency near 70000N (plates/m) by the developed capillary‐HPLC. |
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Keywords: | frit miniaturization stainless steel particle capillary‐HPLC slurry packing |
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