Lactate Biosensor Based on the Adsorption of Polyelectrolyte Stabilized Lactate Oxidase into Porous Conductive Carbon |
| |
Authors: | Vasilis G. Gavalas Nikolas A. Chaniotakis |
| |
Affiliation: | (1) Laboratory of Analytical Chemistry, Department of Chemistry, University of Crete, 71409 Iraklion, Crete, Greece, GR |
| |
Abstract: | In this work the development a lactate biosensor is illustrated. Lactate oxidase is stabilized with the cationic polyelectrolyte diethylaminoethyl-dextran, and the resulting enzyme-polyelectrolyte complexes are physically absorbed into a highly porous and conductive carbon electrode for the construction of the biosensor. The amount of diethylaminoethyl-dextran used is optimized with respect to the sensor sensitivity and stability. Optimum results obtained with enzyme solution containing 0.5% w/v diethylaminoethyl-dextran and 200 U/ml lactate oxidase. The resulting biosensors present increased operational (over 240 hours of continuous polarization) and storage stability (more than 5 months), while the reproducibility was calculated to be better than 5.0% RSD. |
| |
Keywords: | : Lactate biosensor polyelectrolyte porous carbon stability reproducibility. |
本文献已被 SpringerLink 等数据库收录! |