Functionalised benzo[a]phenoxazine dyes as long-wavelength fluorescent probes for amino acids |
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| Authors: | Vâ nia H.J. Frade,Joã o C.V.P. Moura |
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| Affiliation: | a Centro de Química, Universidade do Minho, Gualtar, 4710-057 Braga, Portugal
b Centro de Física, Universidade do Minho, Gualtar, 4710-057 Braga, Portugal |
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| Abstract: | A series of monoreactive functional benzo[a]phenoxazinium chlorides with a carboxyl, hydroxyl, amine or chloromethyl group were used as labels in the preparation of long-wavelength fluorescent amino acid bioconjugates. UV-visible and fluorescence studies of all compounds were carried out in ethanol and water at physiological pH. The absorption and emission of all compounds synthesised were in the range 555-640 nm and 632-681 nm, respectively. |
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| Keywords: | Long-wavelength fluorescent dyes Functionalised probes Benzo[a]phenoxazine Fluorescent labelling Amino acids |
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