How far can we go with structural mass spectrometry of protein complexes? |
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Authors: | Michal Sharon |
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Institution: | 1.Department of Biological Chemistry,Weizmann Institute of Science,Rehovot,Israel |
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Abstract: | Physical interactions between proteins and the formation of stable complexes form the basis of most biological functions.
Therefore, a critical step toward understanding the integrated workings of the cell is to determine the structure of protein
complexes, and reveal how their structural organization dictates function. Studying the three-dimensional organization of
protein assemblies, however, represents a major challenge for structural biologists, due to the large size of the complexes,
their heterogeneous composition, their flexibility, and their asymmetric structure. In the last decade, mass spectrometry
has proven to be a valuable tool for analyzing such noncovalent complexes. Here, I illustrate the breadth of structural information
that can be obtained from this approach, and the steps taken to elucidate the stoichiometry, topology, packing, dynamics,
and shape of protein complexes. In addition, I illustrate the challenges that lie ahead, and the future directions toward
which the field might be heading. |
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