Enhanced Enzymatic Hydrolysis of Lignocellulose by Optimizing Enzyme Complexes |
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Authors: | Mingjia Zhang Rongxin Su Wei Qi Zhimin He |
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Institution: | (1) State Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University, 92 Weijin Road, Nankai District, Tianjin, 300072, China; |
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Abstract: | To enhance the conversion of the cellulose and hemicellulose, the corncob pretreated by aqueous ammonia soaking was hydrolyzed
by enzyme complexes. The saturation limit for cellulase (Spezyme CP) was determined as 15 mg protein/g glucan (50 filter paper
unit (FPU)/g glucan). The accessory enzymes (β-glucosidase, xylanase, and pectinase) were supplemented to hydrolyze cellobiose
(cellulase-inhibiting product), hemicellulose, and pectin (the component covering the fiber surfaces), respectively. It was
found that β-glucosidase (Novozyme 188) loading of 1.45 mg protein/g glucan 30 cellobiase units (CBU)/g glucan] was enough
to eliminate the cellobiose inhibitor, and 2.9 mg protein/g glucan (60 CBU/g glucan) was the saturation limit. The supplementation
of xylanase and pectinase can increase the conversion of cellulose and hemicellulose significantly. The yields of glucose
and xylose enhanced with the increasing enzyme loading, but the increasing trend became low at high loading. Compared with
xylanase, pectinase was more effective to promote the hydrolysis of cellulose and hemicellulose. The supplementation of pectinase
with 0.12 mg protein/g glucan could increase the yields of glucose and xylose by 7.5% and 29.3%, respectively. |
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