反相高效液相色谱法同时测定防晒化妆品中的防腐剂和防晒剂

 用反相高效液相色谱法实现了对化妆品中4种防腐剂（对羟基苯甲酸甲酯、对羟基苯甲酸乙酯、对羟基苯甲酸丙酯、对羟基苯甲酸丁酯）和6种防晒剂（2-羟基-4-甲氧基二苯甲酮-5-磺酸、2-羟基-4-甲氧基二苯甲酮、水杨酸苯酯、对甲氧基肉桂酸辛酯、对二甲基氨基苯甲酸辛酯、水杨酸辛酯）的分离测定。各组分回收率（n=6）为87.2%～106.5%；相对标准偏差（n=6）为1.2%～3.3%。

Determination of 4 Antiseptics and 6 Sunscreen Agents in Cosmetic Products by Reversed-Phase High Performance Liquid Chromatography

A method for the quantitative determination of 4 antiseptics (methyl p-hydroxybenzoate, ethyl p-hydroxybenzoate, n-propyl p-hydroxybenzoate, n-butyl p-hydroxybenzoate) and 6 sunscreen agents (2-hydroxy 4-methoxybenzophenone-5-sulfonic acid, 2-hydroxy 4-methoxybenzophenone, phenyl salicylate, 2-ethylhexyl 4-dimethyl aminobenzoate, 2-ethylhexyl 4-methoxycinnamate, 2-ethylhexyl salicylate) in cosmetic products was developed successfully. It was based on a reversed-phase high performance liquid chromatographic separation under the conditions of isocratic elution with a mixture of methanol-THF-water (30:100:100, V/V) by using a column packed with 10 microns YWG-C18, and the UV detection at 254 nm and 310 nm. The recoveries(n = 6) ranged from 87.2% to 106.5%, and the relative standard deviations(n = 6) were from 1.2% to 3.3%.