光照强度对蛇足石杉共生蓝藻细胞悬浮培养的影响

以蛇足石杉孢子囊为材料,分离出蛇足石杉共生蓝藻细胞,研究光照强度对液体培养的共生蓝藻细胞生长的影响.研究结果表明,在光强500 Lx下,共生蓝藻的生长速率最大;增加光照强度(> 2000 Lx)将抑制共生蓝藻的生长,菌体褪绿变白;在共生蓝藻生长过程中,蓝藻细胞内叶绿素a的含量随着光强的增加而显著减少,这与其在强光照下生物量的降低呈正相关.不同光质实验结果表明,绿光和蓝光条件下蛇足石杉内生藻生长快而红光条件下藻体增殖最慢,这与在绿光和蓝光条件下藻体细胞合成的色素含量增高有密切的关系.实验结论:蛇足石杉共生蓝藻细胞适宜的光照条件是弱光光照,适宜的光质是绿光或蓝光.

Effects of Light Intensity on the Growth of Cyanobacteria Isolated from Huperzia. Serrata (Thunb.) Trevis in Liquid Medium

The symbiotic cyanobacteria were isolated from the sporangium of Huperzia. Serrata (Thunb.) Trevis (H. Serrata). And the effects of light intensity on the growth of cyanobacteria were studied. The results showed that the optimal biomass of cyanobacteria cell was obtained under 500 Lx light intensity. The higher light intensity (> 2000 Lx) repressed the cyanobacteria cell growth which would be faded and turned white. During the growth of symbiotic cyanobacteria, the chlorophyll a content was significantly reduced with the light intensity increased, which were positively correlated with the biomass reduction under higher light intensity. In addition, we found that the biomass and the chlorophyll a content of symbiotic cyanobacteria were higher under green light or blue light than those under red light. In conclusion, the weak light was suitable for the growth of symbiotic cyanobacteria isolated from the sporangium of H. Serrata. And the optimal light quality on the growth of symbiotic cyanobacteria was green light or blue light.