High-performance liquid chromatographic determination of ibuprofen, its metabolites and enantiomers in biological fluids |
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| Authors: | G Geisslinger K Dietzel D Loew O Schuster G Rau G Lachmann K Brune |
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| Institution: | Department of Pharmacology and Toxicology, University of Erlangen-Nürnberg, F.R.G. |
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| Abstract: | An isocratic high-performance liquid chromatographic method to determine racemic ibuprofen (assay I) and its major metabolites (assay II) in biological fluids (plasma, urine, bile) using a conventional reversed-phase column is described. A third assay using beta-cyclodextrin as stationary phase (Cyclobond I) for the separation of the ibuprofen enantiomers is also described. A wavelength of 220 nm was used to monitor the substances. The sensitivity of the method was 0.1 microgram/ml for all three assays. The method was demonstrated to be suitable for stereoselective pharmacokinetic studies of ibuprofen in humans and animals. |
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