Synthesis and Application of Water-Soluble Oxazine Dyes for Detection of PHAs-Producing Bacteria |
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| Authors: | Qing-Hao Liu Jin-Chun Guo Hong-Yu Lu Yan-Nan Guo Hai-Bin Wang Zhi-Yong Hu Hong-Yan Liu Dong-Liang Li-Gong Chen |
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| Institution: | 1.School of Chemical Engineering and Technology,North University of China,Taiyuan,China;2.School of Environment and Safety Engineering,North University of China,Taiyuan,China;3.Institute of Plant Protection,Henan Academy of Agriculture Sciences,Zhengzhou,China;4.School of Chemical Engineering and Technology,Tianjin University,Tianjin,China |
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| Abstract: | Derivatives of oxazine dyes were synthesized on mulitigram scales via efficient synthetic strategies. One practical route was selected to prepare compounds 6, 9 and 10, especially water-soluble compound 6 was obtained in better yield than reported, and compound 10 was insoluble in aqueous media in absence of phenolic-OH. Compounds 3 and 9 were found to be clear pH-dependent between pH?=?4.0 and 10.0, and could be used as acid-base indicators to measure intracellular pH. Compounds 6, 9, 10 all have carboxylic acid functionalities, which could be activated and used to conjugate the dyes to biomolecules. In addition, compounds 6 and 9 with good solubility in aqueous media were used to develop a simple, quick, safe, highly sensitive staining method to detect PHAs-producing bacteria on heat-fixed smears, which was confirmed by fluorescence images of PHAs granules of bacteria. |
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