| Abstract: | Three decadeoxyribonucleotides (d-C-C-A-A-I-A-T-T-G-G, d-C-brC-A-A-G-A-T-T-G-G and d-C-C-A-A-mG-A-T-T-G-G) involving inosine (I), 5-bromocytidine (brC) and 6-O-methylguanosine (mG) were chemically synthesized based on the phosphotriester approach in milligram quantities. The procedures of full protection reaction of these uncommon or modified bases are given in detail. The phosphotriester method was then employed to synthesize all the decamers. It seems the phosphotriester method is applicable to the synthesis of oligodeoxyribonucleotide with these three odd-bases. However, slightly different procedures have to be used while deblocking the fully protected oligodeoxyribonucleotides depending upon the nature of the uncommon or modified base. |
