Quantitation of urapidil and its metabolites in human serum by high performance liquid chromatography |
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Authors: | M Nieder C Dilger R Haerlin |
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Abstract: | The antihypertensive agent urapidil (Ebrantil Byk-Gulden, Konstanz) can be reliably quantitated with three metabolites in human serum using high performance liquid chromatography. Serum is alkalinized and extracted with ethyl acetate. The organic phase is back-extracted with diluted acid. An aliquot is sampled automatically and chromatographed in an optimized combination of mobile and stationary phase. UV-detection at 273 nm allows a quantitation limit of 5 ng/ml for all analytes. Precise handling of exact volumes facilitates external calibration. The coefficient of variation for spiked samples is less than 5% within and less than 7% between studies. Application of the method to experimental and clinical pharmacokinetic studies of urapidil is illustrated. |
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Keywords: | Liquid chromatography HPLC Pharmacokinetics Quantitation in human serum Antihypertensive agents Ebrantil Urapidil |
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