| 水-乙醇溶液中肌红蛋白在琼脂糖膜中的氧化还原和电催化 |
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| 引用本文： | 戴高鹏,刘素芹,黄琳,王启会. 水-乙醇溶液中肌红蛋白在琼脂糖膜中的氧化还原和电催化[J]. 分析试验室, 2008, 27(5): 65-68 |
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| 作者姓名： | 戴高鹏 刘素芹 黄琳 王启会 |
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| 作者单位： | 襄樊学院化学与生物科学系,襄樊,441053;襄樊学院化学与生物科学系,襄樊,441053;襄樊学院化学与生物科学系,襄樊,441053;襄樊学院化学与生物科学系,襄樊,441053 |
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| 摘 要： | 用琼脂糖(Agarose)将肌红蛋白(Mb)固定在玻碳电极(GCE)表面,制备了Mb-Agrose膜修饰电极。在乙醇亲水性有机溶剂与水的混合溶液中,包埋在Agrose中的Mb可以与电极发生直接电子传递。紫外可见光谱表明水-乙醇溶液中琼脂糖膜中的肌红蛋白的构象保持不变。Mb-Agrose膜能快速地催化氯代乙烷(六氯乙烷、五氯乙烷和四氯乙烷等)脱氯,具有较好的稳定性和重现性,可用于这些物质的定量检测。
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| 关 键 词： | 肌红蛋白 电化学 氯代乙烷 |
| 文章编号： | 1000-0720(2008)05-065-04 |
| 修稿时间： | 2007-03-29 |
Redox and electrocatalysis of myoglobin at agarose hydrogel films in aqueous/ethanol solution |
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| DAI Gao-peng,LIU Su-qin,HUANG Lin,WANG Qi-hui. Redox and electrocatalysis of myoglobin at agarose hydrogel films in aqueous/ethanol solution[J]. Chinese Journal of Analysis Laboratory, 2008, 27(5): 65-68 |
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| Authors: | DAI Gao-peng LIU Su-qin HUANG Lin WANG Qi-hui |
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| Abstract: | Myoglobin(Mb) was immobilized on a glassy carbon electrode(GCE) surface by agrose films.Mb entrapped in the agrose films underwent fast direct transferelectron reactions in water-ethanol mixture.UV-Vis absorption(UV-Vis) spectra suggested that the Myoglobin keep their original conformation in the agarose films in water/ethanol mixture.The immobilized Mb is able to dehalogenate chloroethanes(hexachloroet hane,pentachloroet hane,tetrachloroet hane).Stability and reproducibility of the modified electrode could be used to determine the organic chloroe-thanes. |
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| Keywords: | Myoglobin Electrochemistry Chloroethanes |
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