Application ofo-Hydroxyphenylfluorone as a Fluorogenic Indicator to the Determination of Hydrogen Peroxide and Oxidase Substrates Based on the Catalytic Effect of Hemin |
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| Authors: | Qing-Zhi Zhu Xue-Ying Zheng Jin-Gou Xu Feng-Hua Liu Qing-Ge Li |
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| Affiliation: | aResearch Laboratory of SEDC of Analytical Science for Material and Life Chemistry, Department of Chemistry, Department of Biology, Xiamen University, Xiamen, 361005, People's Republic of China;bDepartment of Chemistry, State Laboratory for Tumor Cell Engineering, Department of Biology, Xiamen University, Xiamen, 361005, People's Republic of China |
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| Abstract: | A highly sensitive fluorescence quenching method has been developed for selective determination of hydrogen peroxide based on the catalytic effect of hemin on theo-hydroxyphenylfluorone (a new fluorogenic substrate) and hydrogen peroxide system. Under optimum conditions, the calibration graph was linear over the range 0–1.0 × 10−6mol/liter hydrogen peroxide, with a limit of detection of 8.0 × 10−9mol/liter in a 10-min reaction period. It can easily be incorporated into the determination of biochemical substances that produce hydrogen peroxide under catalytic oxidation by their oxidase. This possibility has been tested for the determination of glucose in human sera as an example. |
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