Fingerprinting Trichoderma reesei hydrolases in a commercial cellulase preparation |
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| Authors: | Vinzant T. B. Adney W. S. Decker S. R. Baker J. O. Kinter M. T. Sherman N. E. Fox J. W. Himmel M. E. |
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| Affiliation: | (1) Biotechnology Center for Fuels and Chemicals, National Renewable Energy Laboratory, 80401 Golden, CO;(2) Biomolecular Research Facility, University of Virginia Medical School, 22908 Charlottesville, VA |
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| Abstract: | Polysaccharide degrading enzymes from commercial T. reesei broth have been subjected to “fingerprint” analysis by high-resolution 2-D gelelectrophoresis. Forty-five spots from 11×25 cm Pharmacia gels have been analyzed by LC-MS/MS and the resulting peptide sequences were compared toexisting databases. Understanding the roles and relationships of component enzymes from the T. reesei cellulase system acting on complex substrates is key to the development of efficient artificial cellulase systems for the conversion of lignocellulosic biomass to sugars. These studies suggest follow-on work comparing induced and noninduced T. reesei cells at the proteome level, which may elucidate substrate-specific gene regulation and response. |
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| Keywords: | Cellulase Trichoderma reesei two-dimensional gel electrophoresis liquid chromatography-mass spectrometry/mass spectrometry |
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