| DNA与表面活性剂相互作用研究(Ⅱ)--基于构象变化的分子水平研究 |
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| 引用本文： | 张剑,敬登伟,谢克昌,张高勇. DNA与表面活性剂相互作用研究(Ⅱ)--基于构象变化的分子水平研究[J]. 化学通报, 2003, 66(12): 799-806 |
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| 作者姓名： | 张剑 敬登伟 谢克昌 张高勇 |
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| 作者单位： | [1]中国日用化学工业研究院表面活性剂国家工程研究中心，太原030001 [2]太原理工大学煤科学与技术教育部重点实验室，太原030032 |
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| 摘 要： | 综述了DNA在稀溶液中与表面活性剂在分子水平上的相互作用的研究进展。介绍了DNA与表面活性剂的两阶段键合过程以及伴生的DNA由线圈向小球的构象变化及其理论解释。同时阐述了相关的对其微观构象变化的研究方法，如热变性实验、荧光光谱、荧光显微技术、动静态光散射等。对DNA与表面活性剂形成的复合物作为基因转染载体的前景及限制因素作了简要介绍。
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| 关 键 词： | DNA 表面活性剂 相互作用 键合等温线 线圈 小球 构象变化 基因转染载体 |
| 修稿时间： | 2002-09-25 |
Studies on the Interaction between DNA and Surfactant(Ⅱ)--Studies at Molecular Level Based on Conformational Transition |
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| Zhang Jian ,,Jing Dengwei,Xie Kechang,Zhang Gaoyong . Studies on the Interaction between DNA and Surfactant(Ⅱ)--Studies at Molecular Level Based on Conformational Transition[J]. Chemistry, 2003, 66(12): 799-806 |
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| Authors: | Zhang Jian Jing Dengwei Xie Kechang Zhang Gaoyong |
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| Affiliation: | Zhang Jian 1,2,Jing Dengwei1,Xie Kechang2,Zhang Gaoyong 1,2* |
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| Abstract: | The progress in the study of the interaction between DNA and surfactant in dilute solution was reviewed.The interaction leads to a two-stage binding process accompanied by the conformational transition of DNA from elongated coil to compact globule.The corresponding theoretical explanation was presented.The relative methods used in the study of the DNA's conformational transition were introduced,including heating-denature test,fluorescent spectrum,fluorescent microscopic technology and dynamic and static light scatterings etc.Besides,the perspective as well as the limiting elements of the complex formed by DNA and surfactant as the carrier of controlled genetic transfer was discussed briefly. |
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| Keywords: | DNA Binding isotherms Coil Globule |
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