Ex Vitro Expansion of Human Placenta-Derived Mesenchymal Stem Cells in Stirred Bioreactor |
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Authors: | Yanqiu Yu Kun Li Chunyu Bao Tianqing Liu Yunan Jin Haiqin Ren Wei Yun |
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Institution: | (1) Department of Pathophysiology, China Medical University, Shenyang, Liaoning, 110001, China;(2) Stem Cell and Tissue Engineering Laboratory, Dalian University of Techonology, Dalian, Liaoning, 116024, China;(3) Shenyang Jinghua Hospital, Shenyang, Liaoning, 110001, China |
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Abstract: | The high demand of human placenta-derived mesenchymal stem cells (hPDMSCs) for therapeutic applications requires reproducible
production of large numbers of well-characterized cells under well-controlled conditions. However, no method for fast hPDMSCs
proliferation has yet been reported. In the present study, the feasibility of using a stirred bioreactor system to expand
hPDMSCs was examined. hPDMSCs were cultured either in stirred bioreactors or in tissue culture flasks (T-flasks) for 5 days.
Total cell density and several parameters of physical microenvironments were monitored in the two culture systems every 24 h.
The maintenance of the antigenic phenotype of hPDMSCs before and after culturing in the stirred bioreactor system was cytometrically
assessed. Data suggested that the physical microenvironment in the stirred bioreactors was much more favorable than that of
the T-flasks. At the end of 144 h culturing, the total cell number was increased 1.73 times from the T-flasks to the stirred
bioreactors. In addition, hPDMSCs could maintain their antigenic phenotype when cultured in stirred bioreactors. These results
provide the initial assessment for large-scale hPDMSCs production using suspension culture bioreactors. |
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