首页 | 本学科首页   官方微博 | 高级检索  
     检索      


A two-dimensional artifact from asynchronous decoupling
Authors:van Ingen Hugo  Vuister Geerten W  Tessari Marco
Institution:Department of Biophysical Chemistry, NSRIM Center, University of Nijmegen, Toernooiveld 1, 6525 ED Nijmegen, The Netherlands.
Abstract:Many heteronuclear NMR experiments employ decoupling to collapse the heteronuclear multiplet, using decoupling schemes with a periodic phase modulation like WALTZ, MLEV, or GARP. Because of the periodic nature of these schemes, cycling sidebands are generated, whose intensity can be strongly reduced by decoupling asynchronously. We show that the most common implementation of asynchronous decoupling on modern spectrometers is such that the cycling sidebands are subjected to a periodic modulation. For multidimensional experiments, this results in ridges that can seriously compromise the quality of the spectrum. Based on our model, the artifact in a 2D (1)H]-(15)N NOE equilibrium experiment is simulated and it is shown that the artifact can be prevented by using synchronous decoupling.
Keywords:Abbreviations: asynchronous decouplingAbbreviations: heteronuclear NOEAbbreviations: cycling sidebandsAbbreviations: broadband decouplingAbbreviations: WALTZ
本文献已被 ScienceDirect PubMed 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号