南北五味子荧光光谱差异的原因

建立了用荧光法快速鉴别五味子和部分含五味子制剂中五味子是属于北五味子还是南五味子的方法，探明了南北五味子荧光光谱差异的主要原因。研究了五味子的五种活性成分五味子醇甲、五味子酯甲、五味子甲素、五味子乙素和安五脂素的荧光性质和五味子以及护肝片乙醇提取液的荧光光谱，并用高效液相色谱测定了供试品五种活性成分的含量。研究表明：发光效率按安五脂素、五味子乙素、五味子甲素和五味子醇甲依次减弱，五味子酯甲不具有荧光性；北五味子的主要荧光物质为五味子乙素，而南五味子的主要荧光物质是安五脂素，它们是导致南北五味子荧光光谱差异的主要原因。用荧光法鉴别南北五味子具有简单、快速和灵敏的特点

Reason of Difference on Fluorescence Spectra between Schisandra and Kadsura

In the paper, a method for the determination of Schisandra and Kadsura was established based on their fluorescence spectra and primary reason of difference on fluorescence spectra between Schisandra and Kadsura was found out. Fluorescence properties of schisandrol A, schisantedrin A, deoxyschizandrin, g-schisandrin, anwulignan and ethanolic extract of Schisandra chinensis and Hugan tablets, were studied by fluorospectrophotometry and contents of the five substances were determined in Schisandra chinensis and Hugan tablets by high pertormance liquid chromatography. The results showed that the emission efficiency was decrescent from anwulignan, g-schisandrin, deoxyschizandrin to schisandrol A, however, the emission and excitation spectra of schisantherin A couldn't been obtained in the same condition. g-Schisandrin was primary fluorescence substance in Schisandra, however, anwulignan was primary fluorescence substance in Kadsura, so there was obvious difference on fluorescence spectra between Schisandra and Kadsura. The fluorospectrophotometry was used for the determination of Schisandra and Kadsura, the method was quick, simple and sensitive.