智能手机微孔板分析仪的开发与HSA的定量检测

研究开发了基于智能手机的便携式96微孔板检测技术与仪器,并利用所开发仪器实现了人血清白蛋白(HSA)的高通量快速定量检测。该文首次通过设计大孔径聚焦透镜和均匀面光源,利用智能手机实现了对通用96微孔板的全范围准确成像,结合开发的手机应用程序可实现对检测样本的自动分析。所开发分析仪对HSA标准样品的检测结果与酶标仪检测结果具有高度的线性关系,相关系数为0.989 3,证明其具有良好的准确性与可靠性。开发的分析仪的检测范围为21.45～60.06 g/L,对病人血清样品中HSA的检测结果与医院使用的生化分析仪检测结果一致,证明分析仪可用于临床中HSA的检测。该仪器具有成本低和体积小的特点,可推广至经济不发达地区、基层医疗机构和普通用户家中,对疾病的现场快速诊断具有重要意义。

Development of a Smartphone based Microplate Analyzer and Its Quantitative Detection for Human Serum Albumin

A portable microplate analyzer based on a smartphone was developed for rapid high throughput quantitative detection of human serum albumin(HSA).A lens with large aperture and a LED with uniform plate were firstly used in this article to assist the smartphone to achieve a uniform imaging for the whole 96 microplate.A custom designed smartphone application was used for automatic analysis.There was a good linear relationship between results obtained with this analyzer and those of microplate reader for detection of HSA standard samples,with a correlation coefficient of 0.989 3 and the detection range of 21.45-60.06 g/L,which proved that the developed analyzer has great accuracy and reliability.In addition,the detection results of human serum samples also showed good agreement between the clinical analyzer and the proposed one,revealing that the developed analyzer could also be used for clinical HAS detection.With the characteristics of low cost and being small in size,the analyzer is suitable for those underdeveloped areas,primary care institutions and ordinary families,and is of great significance for rapid on site diagnosis on the disease.