Multifocal multiphoton microscopy based on a spatial light modulator |
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| Authors: | Y. Shao W. Qin H. Liu J. Qu X. Peng H. Niu B. Z. Gao |
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| Affiliation: | 1. College of Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen, 518060, China
2. Department of Bioengineering and COMSET, Clemson University, Clemson, SC, 29634, USA
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| Abstract: | We present a new multifocal multiphoton microscope that employs a programmable spatial light modulator to generate dynamic multifocus arrays which can be rapidly scanned by changing the incident angle of the laser beam using a pair of galvo scanners. Using this microscope, we can rapidly select the number and the spatial density of focal points in a multifocus array, as well as the locations and shapes of arrays according to the features of the areas of interest in the field of view without any change to the hardware. |
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