一种新的转铁蛋白压电免疫传感器的研究

报道了一种基于等离子体聚合膜、结合聚电解质设计的转铁蛋白压电免疫传感器.采用辉光放电等离子体聚合技术,在石英晶体上沉积一层正丁胺聚合膜,再在膜上自组装一层易再生的、带负电的聚电解质,调节抗体溶液的pH值使其带正电,经静电吸附包被抗体后用以测定抗原.探讨了自组装聚电解质的浓度和自组装时间,抗体的包被浓度、包被时间和pH值以及免疫反应的酸度、温度及响应频率与时间的关系等实验条件的影响.考察了传感器的灵敏度、选择性、重现性和再生性能.用传感器测定人血清中转铁蛋白的线性范围为0.10～12.65μg/mL.将其用于实际样品中转铁蛋白的测定,结果与酶联免疫法基本一致.

A Novel Piezoelectric Immunosensor for the Determination of Transferrin

Based on plasma-polymerized film(PPF) and polyelectrolyte, a novel piezoelectric immunosensor is developed for the determination of transferrin in human serum. The approach begins with the preparation of PPFon the surface of quartz crystal microbalance(QCM) by the glow-discharge. Through strong electrostatic attraction, a negative-charged polyelectrolyte which serves as a removable layer for the QCMreproduction is then self-assembled on the film, and positive-charged antibodies which specifically react with antigens are subsequently immobilized on the self-assembled layer by controlling the pHvalue. The effects of experimental conditions including the self-assembly of polyelectrolyte, the incubation of transferrin antibodies and the test of immunoreaction are discussed, respectively. In addition, the sensitivity, selectivity, regeneration and reproducibility of the immunosensor are investigateDIn detail. Under the optimal experimental conditions, the QCM immunosensor can be used to determine quantitatively transferrin in human serum with the linear range of 0.10-12.65 μg/mL. It is found that the results of clinical samples by this developed method are identical to those by the ELISAmethod.