| Protein conformation in solution by three-dimensional fluorescence spectrometry |
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| 作者姓名： | 鄢远 许金钩 陈国珍 |
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| 作者单位： | Department of Chemistry，Nanchang University，Nanchang 330047，China.，Department of Chemistry，Xiamen University，Xiamen 361005，China，Department of Chemistry，Xiamen University，Xiamen 361005，China |
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| 基金项目： | Project supported by the National Natural Science Foundation of China |
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| 摘 要： | The conformations of bovine serum albumin (USA) and egg albumin (EA) in solution and their conformation changes under different conditions were studied by using three-dimensional fluorescence spectrometry (TDFS) such as three-dimensional fluorescence (TDF) spectra and three-dimensional fluorescence polarization (TDFP) spectra with tryptophan residues in protein molecules as an intrinsic fluorescent probe. The results show that the microenvironment of tryptophan residues of protein molecules in various solutions can be directly indicated and TDFS is an effective tool for studying protein conformation in solution. Meantime, some valuable results were obtained.
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Protein conformation in solution by three-dimensional fluorescence spectrometry |
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| YAN Yuan XU Jingou CHEN Guozhen.Protein conformation in solution by three-dimensional fluorescence spectrometry[J].Science in China(Chemistry),1996(5). |
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| Authors: | YAN Yuan XU Jingou CHEN Guozhen |
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| Institution: | YAN Yuan XU Jingou CHEN Guozhen Department of Chemistry,Nanchang University,Nanchang 330047,China. |
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| Abstract: | The conformations of bovine serum albumin (USA) and egg albumin (EA) in solution and their conformation changes under different conditions were studied by using three-dimensional fluorescence spectrometry (TDFS) such as three-dimensional fluorescence (TDF) spectra and three-dimensional fluorescence polarization (TDFP) spectra with tryptophan residues in protein molecules as an intrinsic fluorescent probe. The results show that the microenvironment of tryptophan residues of protein molecules in various solutions can be directly indicated and TDFS is an effective tool for studying protein conformation in solution. Meantime, some valuable results were obtained. |
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| Keywords: | three-dimensional fluorescence spectrometry bovine serum albumin egg albumin protein conformation |
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