Chromatoid body: remnants of nucleolar proteins during spermatogenesis in triatomine (Heteroptera, Triatominae) |
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| Authors: | Silistino-Souza Rosana Peruquetti Rita Luiza Taboga Sebastião Roberto Vilela de Azeredo-Oliveira Maria Tercília |
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| Institution: | 1. Department of Biology, São Paulo State University – UNESP/IBILCE, Rua: Cristóvão Colombo, 2265, Jardim Nazareth, 15054-000 São José do Rio Preto, SP, Brazil;2. Center of Health Sciences, Sacred Heart University – USC, Bauru, SP, Brazil;1. Department of Computer Science and Statistics (DCCE), São Paulo State University (UNESP), São José do Rio Preto, SP, Brazil;2. Faculty of Computation (FACOM), Federal University of Uberlândia (UFU), Uberlândia, MG, Brazil;3. Center of Mathematics, Computing and Cognition, Federal University of ABC (UFABC), Santo André, SP, Brazil;4. Federal Institute of Triângulo Mineiro (IFTM), Ituiutaba, MG, Brazil;5. Transdisciplinary Center for Study of Chaos and Complexity (NUTECC), São José do Rio Preto Medical School, São José do Rio Preto, SP, Brazil;6. Kidney Transplant Surgical Service, Base Hospital, Fundação Faculdade Regional de Medicina (FUNFARME), São José do Rio Preto, SP, Brazil;7. Pathologic Anatomy Service, Base Hospital, Fundação Faculdade Regional de Medicina (FUNFARME), São José do Rio Preto, SP, Brazil;1. Department of Drug Discovery & Biomedical Sciences, Medical University of South Carolina, Charleston, SC 29425, United States;2. The Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning Province, China;3. Department of Biochemistry & Molecular Biology, Medical University of South Carolina, Charleston, SC 29425, United States;4. Ralph H. Johnson Veterans Affairs Medical Center, Charleston, SC 29403, United States;3. Murdoch Childrens Research Institute, Royal Children''s Hospital, Melbourne, Victoria 3052;4. Department of Paediatrics, University of Melbourne, Royal Children''s Hospital, Melbourne, Victoria 3052, Australia;1. State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing, P. R. China;2. National Engineering Research Center for Protein Drugs, Beijing, P. R. China;3. National Center for Protein Sciences at Beijing, Beijing, P. R. China;4. School of Life Sciences, Tsinghua University, Beijing, P. R. China;5. Clinical Medical Institute of General Hospital of Xinjiang Military Area, PLA, Urumqi, Xinjiang, P. R. China;6. Affiliated Cancer Hospital of Guangxi Medical University, Nanning, Guangxi, P. R. China;7. National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences; School of Basic Medicine, Beijing, P. R. China;8. Department of Gastroenterology and Hepatology, Zhongshan Hospital, Fudan University, Shanghai, P. R. China;9. Department of Experimental Research, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong, P. R. China;10. Center for Genetics and Genomics Analysis, Genesky Biotechnologies Inc., Shanghai, P. R. China;11. Department of Infectious Diseases, Affiliated Youan Hospital, Capital University of Medical Science, Beijing, P. R. China;12. Interventional Radiology Department of Affiliated Tumor Hospital of Guangxi Medical University, Nanning, Guangxi, P. R. China;13. Department of Urology and Nephrology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, P. R. China;1. BADJI Mokhtar University, Faculty of Sciences, Biochemistry Department, Annaba, Algeria;2. Houari Boumediene Sciences Technology University, Faculty of Biological Sciences, Arid Zones Research Laboratory (LRZA), Algiers, Algeria;3. University Hospital, Genetic Department, F-14033 Caen, France;4. University Caen, Basse Normandie, France;5. INSERM U1075, France;1. Department of Pharmacology, University of Michigan, Ann Arbor, Michigan;2. Department of Physics and LSA Biophysics, University of Michigan, Ann Arbor, Michigan |
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| Abstract: | In this study, we analyzed fibrillarin nucleolar protein expression in CBs of spermatogenic cells from testicular follicles of Triatoma sordida and Triatoma infestans. In the structural and ultrastructural analysis, it was used impregnation by silver ions, immunocytochemistry, immunofluorescence and transmission electron microscopy using antibodies against fibrillarin. Regarding the results, the fibrillarin nucleolar protein marked the nucleus and some cytoplasmic spots of germ cells during spermatogenesis in triatomines. These data suggest that fibrillarin could be a constituent of the CB that was most likely derived from nucleolar fragmentation. This is the first time that fibrillarin protein expression has been shown in the CB during spermatogenesis progression in triatomines. The knowledge regarding CB constituents may help to expand the understanding of the physiological role of this structure and the role that it plays in the reproductive biology of triatomines, which are vectors of Chagas Disease. |
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