A new method for determination of buformin in plasma and urine by ion-paired reversed-phase HPLC with ultraviolet detection |
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| Authors: | Yamamoto Takehito Kusama Makiko Matsuno Kumi Sugiyama Erika Yamada Yasuhiko Iga Tatsuji |
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| Institution: | Department of Pharmacy, The University of Tokyo Hospital, Faculty of Medicine, The University of Tokyo, 3-1 Hongo 7-chome, Bunkyo-ku, Tokyo 113-8655, Japan. |
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| Abstract: | Buformin is a widely used as an antidiabetic agent but its renal excretion is still controversial. A new HPLC method with ultraviolet (UV) detection for the determination of buformin in plasma and urine has been developed. After protein precipitation or dilution, buformin and internal standard phenformin were resolved on an octadecyl silica column and detected by UV detection at 233 nm. Intra- and inter-day coefficients of variation were <9%. The limit of quantification was around 0.05 micro g/ml for plasma and 2.5 micro g/ml for urine. |
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